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	Provedor de dados BABT (1) BJID (1) Autor Benati,Fabrício José (2) Linhares,Rosa Elisa Carvalho (2) Albuquerque,Daniele (1) Czernisz,Érika da Silva (1) Freitas,José Felipe Eliseu (1) Hashimoto,Newton (1) Kerntopf,Gustavo Franthesco (1) Lauretti,Flávio (1) Nozawa,Carlos M (1) Nozawa,Carlos Mitihiko (1) Pietruchinski,Eduardo (1) Romanin,Priscila (1) Santos,Norma (1) Mais... Palavra-chave Determination (1) Enzyme immunoassay (1) Genotyping (1) Hospitalized patients (1) Poliovirus antigen (1) Rotavirus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 2007 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Detection and characterization of human rotavirus in hospitalized patients in the cities of Ponta Grossa, Londrina and Assai - PR, Brazil BJID Nozawa,Carlos M; Kerntopf,Gustavo Franthesco; Czernisz,Érika da Silva; Albuquerque,Daniele; Romanin,Priscila; Freitas,José Felipe Eliseu; Santos,Norma; Benati,Fabrício José; Pietruchinski,Eduardo; Linhares,Rosa Elisa Carvalho. Acute diarrheal disease is still one of the major public health problems worldwide. Rotaviruses (RV) are the most important viral etiologic agents and children under five years of age are the target population. OBJECTIVE: To investigate the rate of RV infection in hospitalized patients due to acute diarrhea in the cities of Ponta Grossa, Londrina and Assai - Paraná. METHODS: Latex agglutination (LA); immunochromatography (ICG); polyacrylamide gel electrophoresis (PAGE) and negative staining electron microscopy (ME) tests were used to detect the virus. For the genotyping, RT-PCR and RT-PCR-ELISA were used, respectively, for NSP4 and VP4/VP7. RESULT: Out of 124 samples there were 69 positive stool samples for RV, for at least one of the used tests, 67 of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Determination; Hospitalized patients; Genotyping. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600002 Development of an enzyme immunoassay for poliovirus antigens BABT Hashimoto,Newton; Benati,Fabrício José; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Nozawa,Carlos Mitihiko. An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme immunoassay; Poliovirus antigen. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005 Registros recuperados: 2 Primeira ... 1 ... Última

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